This is all good stuff from N2! As for cleaning bottles, I recommend rinsing with the hottest water possible to open up the pores of the bottle to get as much whatever out as possible. Then rinse well with rubbing alcohol. Then piss in the bottle. Let this sit a couple days. Dump it out and rinse with hot water again. Then repeat the piss and rinse process a few more times. As many as you feel it takes. Only piss and hot water from then on. Keep this bottle in a special place just for subbing. Do this to as many bottles as you want. I also recommend a glass bottle as the glass acts as an insulator and helps to hold the temp longer. Also, if you have to, you can apply a little last minute heat to it with a lighter in the collection room. Don't use a torch lighter though, as it may crack the glass. Yes it has happened to me, but I was experimenting at home just to see what would happen. Glass bottles are hard to find anymore. I actually use bottles from an energy drink called Bacchus-D. You can find it at any Korean grocer or asian market. Also on Amazon. Get a whole box. 10 bottles in a box....and it tastes good too. The bottles have metal caps, but I like to substitute with good quality plastic caps from soda or water bottles (not the cheap thin caps on most water bottles though). The plastic cap doesn't make any noise in the collection room while unscrewing it. Also, you can buy a cheap baby bottle warmer to heat your sample if this would be feasible for your situation, but be careful, because it can heat it way too hot before you know it. One thing with the glass bottles holding the heat, it will take longer to cool it back down. Running under hot water or dunking into a cup of hot water or coffee does a wonderful job too. Good luck! Oh, one more thing, don't freeze glass bottles.....they WILL crack.