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Old 05-07-2009, 01:54 AM   #1
masterjam77
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I don't understand half of this stuff, but it sure sounds convincing. Interesting stuff. I am still confused on how you would practically use these methods without your urine looking or testing suspicious, but if you can trust anyone about this stuff, its probably a chemist or doctor.
article below from medscape today:: thanks from the mods for the heads up.
Common Household Chemicals as Urinary Adulterants

People try to beat drug testing by adding adulterants to urine specimens. Several adulterants can cause false-negative results in drug testing by immunoassays. Common adulterants for masking drug testing are as follows: (1) table salt, (2) household vinegar, (3) liquid laundry bleach, (4) concentrated lemon juice, (5) goldenseal tea (produces dark urine), and (6) eyedrops.
Although FPIA is less subject to interference from adulterants than the EMIT assay, some interference has also been reported with FPIA. Sodium chloride caused negative interference with all drugs tested by EMIT and a slight decrease in measured concentrations of benzodiazepines by FPIA. Sodium bicarbonate caused false-positive results with an EMIT opiate assay and with PCP testing by FPIA. Hydrogen peroxide caused false-positive benzodiazepine results by FPIA.[21] Uebel and Wium[22] studied the effects of household chemicals (sodium hypochlorite, Dettol [chloroxylenol], glutaraldehyde, Pearl hand soap, ethanol, isopropanol, and peroxide) on cannabis and methaqualone test results using EMIT assays. Most of the agents tested interfered with the test results, and the greatest effect was observed with glutaraldehyde and Pearl hand soap for methaqualone (false-negative). Dettol and Pearl hand soap also caused false-negative results in cannabis tests. Addition of isopropanol, ethanol, and peroxide invalidated a methaqualone test result.[22]
Schwarzhoff and Cody[23] studied the effect of 16 adulterating agents (ammonia-based cleaner, L-ascorbic acid, eyedrops, drain opener, goldenseal tea, lemon juice, lime solvent, liquid bleach, liquid hand soap, methanol, sodium chloride, tribasic potassium phosphate, toilet bowl cleaner, white vinegar, ionic detergent, and whole blood anticoagulated with EDTA) on analysis of urine by FPIA for abused drugs. They tested these adulterating agents at a 10% by volume concentration of urine with the exception of goldenseal tea because of its insolubility. For goldenseal tea, 1 capsule was suspended in 60 mL of urine. Of 6 drugs tested (cocaine metabolites, amphetamines, opiates, PCP, cannabinoid, and barbiturates), the cannabinoid test was most susceptible to adulteration. Approximately half of the agents tested (ascorbic acid, vinegar, bleach, lime solvent, eyedrops, and goldenseal tea) caused false-negative results. Cannabinoid and opiate assays were susceptible to bleach, and actual degradation of THC was confirmed by GC-MS. The PCP and benzoylecgonine (the metabolite of cocaine) analyses were affected by alkaline agents.[23] Baiker et al[24] reported that hypochlorite (a common ingredient of household bleach) adulteration of urine caused a decreased concentration of THC as measured by GC-MS. A false-negative result was also observed with the FPIA screen and the Roche Abuscreen (Roche Diagnostics, Indianapolis, IN).[24] Another report described adulteration of urine specimens with denture cleaning tablets.[25]
The ability of eyedrops to cause false-negative drug test results in the screening phase of the analysis is troublesome because the presence of components of eyedrops in adulterated urine cannot be detected by routine specimen integrity testing or routine urine analysis. Pearson et al[26] studied in detail the effect of eyedrops on drugs-of-abuse testing and the mechanism by which components of eyedrops produce false-negative drug testing results. Eyedrops are effective in causing false negative results in the analysis of the THC metabolite, THC-COOH. GC-MS analysis showed that there was no modification in the structure of the THC metabolite by the components of eyedrops. At low concentrations of eyedrops, the false-negative cannabinoid result was due to the benzalkonium chloride ingredient of eyedrops. Eyedrops decreased the THC assay results in EMIT/drugs-of-abuse assays and Abuscreen, although eyedrops had no effect on the glucose-6- phosphate dehydrogenase drug conjugate used in the EMIT assay. Results of the ultrafiltration studies with eyedrops suggest that the THC metabolite partitions between the aqueous solvent and the hydrophobic interior of benzalkonium chloride micelles, thus reducing the availability of THC metabolite in antibody-based assays.[26] Eyedrops and analgesic heat rub ointment can also cause false-negative drug test results with sweat testing.[27] Components of eyedrops in urine may be detected by using high-performance liquid chromatography combined with UV detection at 262 nm, a method originally developed for analysis of ophthalmic formulations.[28]

Last edited by masterjam77 : 05-07-2009 at 02:20 AM. Reason: broken link
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