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beck

SEC3 - i'm somewhat new to the board and have been reading some of your replies....do you have a background in testing?

I just read the thread about the Quest test with the 10-panel. I just came from a pre-employment test and the paperwork they gave me had something similar. They listed that they are using a 35693N SAP 8-50 w/NIT. What exactly does that mean? I was concerned because next to the marijuana category is said GC/MS. I was hoping it was only an EMIT. From your previous post, I gathered that they will only do a GC/MS if the initial test is positive.

I'm clean for 49 days as of today. I took a home test two days ago and it came up negative and that was obviously with a 50ng cut-off. I'm hoping this is the same.
Thanks for your help.

 

dankitydank

You missed a few things to add though. Whats your weight and metabolism? He or anyone couldn't make a guess to whether you passed or not. If you are a thin dude with a high metabolism, chances are you passed. If your a big guy... then thats another issue.

Sorry sec3, kind of jumped in on your question.

Sec

It is all right, I don't mind. Even with weight and metabolism listed, it is only a guess whether someone will pass or not and fortunately, beck only wants to know what the code for his test means and if it is the same cutoff as his home test.

They are testing for 8 different drugs

SAP = Substance Abuse Panel

Yes, they almost always (for employment almost never do they go straight to the GC/MS) do the EMIT, and need a non-negative reading from the EMIT before sending to the GC/MS.

It is the same for marijuana as the home tests. I am guessing that this was sent to Quest Diagnostics (from the format of the test code).

I'm just about as positive as I can be without giving you a 100%, that you will pass this test if you didn't drink too much fluid and come up diluted.

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nerphroll

Does that mean that a non-negative result, such as a dilute, gets sent to the lab for GC/MS? My understanding was that the only non-negative results that get sent for GC/MS were "positives". That is, tests for which the indicator for the absence of THC doesn't succeed..

I'm confused.

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Sec

The EMIT that I was speaking of is done at the laboratory.

We, the collection facility, sometimes, for some employers, do an instant cup type of test. If it indicates abnormal for adulterants or non-negative for a banned substance, we will forward that specimen to a laboratory for confirmation. If the instant test shows normal for the integrity panel and negative on the test panel, the specimen is determined to be negative and the employer is notified with no laboratory involved. Not all employers order instant testing for their employees, and not all employers want 100% of the tests to go to the lab.

When a specimen is sent to a lab, the laboratory also runs an emit test on the specimen. If, at the lab, the specimen is found to be abnormal for specific gravity, pH, creatinine, or oxidents, or the specimen shows non-negative for a banned substance, the lab will then put the specimen in the GC/MS machine to get quantitative levels.

Did this help or further confuse you.

 

nerphroll

I think it helped. So what you're saying is that for those tests that end up getting sent to the lab, even if it's dilute, it will still be tested by GC/MS, but if the test is done as an instant at the testing facility, a dilute is usually just stopped there.
Thanks.

Sec

almost, but not quite.

think of it as two separate places, two separate test sessions.

At the lab, 100% of the tests are given an emit. If they pass with no problem, then that is it. If there is a problem, it is off to the GC/MS for the specimen.

At the collection facility, specimens have two options. Either they are subject to an instant test at the collection facility or they are not subject to an instant test and are sent straight to the lab.

If they are subject to an instant test at a collection facility, if the specimen tests negative and no integrity panels are abnormal, that is it. The specimen is deemed to be negative and the test process ends without the specimen being sent to a lab.

If the instant test shows abnormal on the integrity panel (which includes oxidents, specific gravity and pH), indicating a diluted or non-human urine specimen or the test panel shows non-negative for a banned substance, the specimen is packaged for shipment to a laboratory.

If the specimen is not subject to instant testing at the collection facility, but rather sent straight to a lab or if a specimen is forwarded to a lab after an abnormal integrity panel or non-negative test panel: At the lab, the specimen is again screened. If the lab screening shows non-negative for a banned substance or abnormal for any of the integrity checks (including specific gravity, oxidents, pH, creatinine and nitrates), the specimen is sent to the GC/MS for confirmation and quantitative levels of the banned substance, creatinine, nitrates, etc.

Usually, when we do an instant test at the collection facility, and it shows abnormal or non-negative, we do not let the lab know that we have previously tested the specimen.

lew scannon

Actually, the analytical methods used to determine the validity of a urine sample is quite different from those used to test a urine sample for drugs. This is because of the wide variety of adulterants that may be used by donors to tamper with a specimen.

The following methods are among those used to conduct initial and confimatory validity tests (My apologies if the wording seems somewhat esoteric):

Colorimetry - An analytical procedure based on comparison of the color developed in a solution of a test material with that in a standard solution and quantitated on the basis of the absorption of light. In a colorimetric test method, reagents are added to a sample of urine and a reaction occurs with the analyte of interest, producing a color. Because the intensity of the color is related to the analyte's concentration, the concentration of the analyte is determined by visually measuring the color or electronically measuring the intensity of light at selected wavelengths (i.e., spectrophotometry). Creatinine concentration and pH may be determined using colorimetric tests.

Refractometry - A refractometer measures indices of refraction. The index of refraction is the ratio of electromagnetic radiation in a vacuum to its velocity in the medium of interest. A urine specific gravity refractometer is used to determine the amount of solute (i.e., urinary total solids) in the urine by measuring the index of refraction. An instrument manufacturer applies a formula to convert refractive indices to urine specific gravity values which are displayed by the refractometer. Certified laboratories are required to use refractometers that report and display specific gravity to four decimal places.

Characteristic Immunoassay Drug Test Responses - These are exhibited by some adulterants using some reagents. This enables laboratories to develop criteria for initial drug test data to identify a specific adulterant (e.g., glutaraldehyde). If these responses are validated by a laboratory for a specific adulterant, the laboratory may accept the abnormal drug test readings as the initial test for that adulterant. The laboratory must validate the immunoassay tests for this use and must analyze required controls in the same batch (i.e., a control with the adulterant and a control without the adulterant) to document that the adulterant produces those test responses. For the confirmatory test, laboratories must use a definitive method for identifying the adulterant (e.g., GC/MS for glutaraldehyde).

Hope that helps a little


Source: http://dwp.samhsa.gov/DrugTesting/Fi...ary%202005.pdf