Dilution and aspirin-- the technique. - Page 2

Smokalicious · 05-08-2003, 02:48 AM

by dilution do you just mean drinking a lot of water?

S2 · 05-09-2003, 12:39 PM

S2 · 05-16-2003, 05:53 PM

Decreased signal in Emit assays of drugs of abuse in urine after ingestion of aspirin: potential for false-negative results.

Wagener RE, Linder MW, Valdes R Jr.

Department of Pathology, University of Louisville School of Medicine, University of Louisville Hospital, KY 40292.

During routine drug analysis with the Syva d.a.u. Emit immunoassays we observed a high frequency of urines with lower rates of changes in absorbance (delta A R) than the rate for a drug-free urine calibrator. Many of these urines contained salicylates. Among 40 urines with apparent salicylate concentrations between 15 and 420 mg/dL tested for benzoylecgonine (BE), 20 had delta A R < -4 (range +2 to -28 mA/min). The rates decreased with increasing salicylate: delta A R = -0.057 x (salicylate, mg/dL) -0.22 mA/min (r = 0.85, n = 40, P < 0.01). Urines from 100 control subjects (no salicylate) had mean +/- SD delta A R values of -1.05 +/- 2.2 mA/min (range +3 to -7; only two were < -4 mA/min). Although direct addition of salicylic acid (200 mg/dL) to urine specimens did not reproduce the negative bias, ingestion of aspirin (acetylsalicylic acid) did by -0.09 mA/min per 1 mg/dL (72.4 mumol/L) salicylate. Negative biases observed for other Emit d.a.u. assays after salicylate ingestion lead us to conclude that ingestion of therapeutic doses of aspirin may cause false-negative results for drug screens in urines by this technology.

PMID: 8149618 [PubMed - indexed for MEDLINE]

S2 · 05-18-2003, 11:58 AM

cant quite figure our their absorbtion units but what this says to me is that the samples containing aspririn do trigger false negative but they also behave differently than a "normal" clean sample i.e.' : "...lower rates of changes in absorbance (delta A R) than the rate for a drug-free urine calibrator..."

This means that there may be a method of detecting the aspirin adulteration down the road-- this is what I am on the look out for.

S2 · 05-28-2003, 06:54 PM

Quote:

Originally posted by Switch420
You've explained how the aspirin helps, but what about the rolaids?

Quote:

Originally posted by S2

I also take a half dozen Tums or rolaids

The reason I do the above is to consume extra salts and minerals in the hopes that as I dilute my urine I am keeping my specific gravity up in case it is checked-- a low specific gravity, which will occur if you flush you system with excess water and it is a means of detecting dilution.

The rolaids and the salt and gatorade are to try to keep the specific gravity up-- drinking pure h20 definitely lowers SG, dont know if the salt/calcium does but I figure it cant hurt

choke-a-toke · 08-03-2004, 01:24 AM

I know you said this method has worked for you and others, but what I need to know is; How long did you stop smoking before you started your asprin/dilution method? I smoked sunday the 1st. and plan on not smoking again till after the test wich will be on the 10th. However, I want to smoke now. Also, what kind of smoker would you consider yourself as, Light or Heavy?

S2 · 08-04-2004, 02:24 PM

Quote:

Originally Posted by choke-a-toke

I know you said this method has worked for you and others, but what I need to know is; How long did you stop smoking before you started your asprin/dilution method? I smoked sunday the 1st. and plan on not smoking again till after the test wich will be on the 10th. However, I want to smoke now. Also, what kind of smoker would you consider yourself as, Light or Heavy?

I smoke about 2 grams a day-- I have used this techniqe after abstaining anywhere from 2 weeks to 3 weeks and was still testing positive on home tests

GanjConsultant · 08-07-2004, 05:34 PM

I work for a large consulting firm in DC. I recently had to take a urine test in order to get security clearance for the project I'm currently working on. I wanted to thank S2 for his post on Dilution but wanted to let all of you know that there is no need to over saturate your salt levels as much as he recommends. Gatorade has more than enough salt naturally in it to replenish the salt levels in your body. In fact most trainers would recommend 1/2 water to 1/2 Gatorade as the best mixture to recover from the normal loss of salts and electrolytes caused by strenuous activities. The reason for this is that Gatorade has more salt and electrolytes in it than is needed and the excess is unnecessarily removed by your liver. Thus if you drank pure Gatorade to dilute in conjunction with the other ingredients, you wouldn't have any problem passing the urine integrity tests. No need to make people puke from such an awful tasting mixture.

I would also recommend eating a meal about 3-4 hours before the test so that you are getting naturally generated vitamins, minerals, proteins, carbohydrates, and fats going through your liver on top of the other things you’re doing. Stay hydrated at normal levels up until 2 hours before the test and then follow S2's liquid intake recommendations. Finally, I would recommend taking a multivitamin about 1 hour before the test.

This worked for me and I hope it can help you pass these pointless tests. Corporate pot smokers unite

mapache · 09-20-2004, 10:15 PM

Hello from Mexico!!

Yesterday I filled to cups and took them to the lab. I made the firts test at 4:00 pm and the second one at 5:00pm. I drank lots of water all day long and one hour before the second test ( right after the first one ) I took 4 aspirins.

The results were as following:

A) With dilution: 51.3ng/mL
B) After aspirin: 63.5ng/mL

The lab's limit was 50 ng/mL

I DID NOT mixed up the cups. Each one was labeled right after having it filled.

I had been smoking every day ( I'm thin and work out 3 times per week ) for the last couple of weeks so i didn't expect much from the tests but since the results went so weird I decided to post them.

My questions are:

1- If my results were ( lets say ) 49.50 ng/mL and the lTHC limit were 50 ng/mL, what would that mean for the tester? Is it like " you are not over the limit but you have THC so either way you smoked!" ?

2- What could explain the difference of 10 ng/mL from test A to test B?

3- Should the Dilution and Aspirin method should work for very frequent users if it is done right?

Thanks a lot and congratulations for this amazing effort.

Mapache

ch0ng0 · 09-23-2004, 03:32 AM

I would not worry about SG unless there is a dispute and you are under
additional scrutiny.

SG tests are very expensive and not commonly used.

Creatine levels and color are most often used.

Vitiams make your pee neon yellow ....so be shure to dilute with by drinking a
pint of water. I drink a pint of water every 15 min for two hours before the test. I use detoxify quick flush.....its worked around 15+ times....The pills have creatine and B2 I also take 3 asprin evey 15min for an hour before the test.

Also bring something to pee in when your in your car.

05-08-2003, 02:48 AM	#1
Smokalicious Jr. Member Join Date: Mar 2003 Posts: 167 Grams: 2,534.05 Thanks: 0 Thanked 0 Times in 0 Posts Stock Portfolio Total Value: 0.000 Gain/Loss: 0.000%	by dilution do you just mean drinking a lot of water? __________________ Role it up, light it up, smoke it up, inhale, exhale..

05-09-2003, 12:39 PM	#2
S2 Just another bonehead Join Date: Dec 2000 Posts: 1,096 Grams: 3,294.00 Thanks: 11 Thanked 24 Times in 15 Posts Stock Portfolio Total Value: 0.000 Gain/Loss: 0.000%	yes

05-16-2003, 05:53 PM	#3
S2 Just another bonehead Join Date: Dec 2000 Posts: 1,096 Grams: 3,294.00 Thanks: 11 Thanked 24 Times in 15 Posts Stock Portfolio Total Value: 0.000 Gain/Loss: 0.000%	FYI Decreased signal in Emit assays of drugs of abuse in urine after ingestion of aspirin: potential for false-negative results. Wagener RE, Linder MW, Valdes R Jr. Department of Pathology, University of Louisville School of Medicine, University of Louisville Hospital, KY 40292. During routine drug analysis with the Syva d.a.u. Emit immunoassays we observed a high frequency of urines with lower rates of changes in absorbance (delta A R) than the rate for a drug-free urine calibrator. Many of these urines contained salicylates. Among 40 urines with apparent salicylate concentrations between 15 and 420 mg/dL tested for benzoylecgonine (BE), 20 had delta A R < -4 (range +2 to -28 mA/min). The rates decreased with increasing salicylate: delta A R = -0.057 x (salicylate, mg/dL) -0.22 mA/min (r = 0.85, n = 40, P < 0.01). Urines from 100 control subjects (no salicylate) had mean +/- SD delta A R values of -1.05 +/- 2.2 mA/min (range +3 to -7; only two were < -4 mA/min). Although direct addition of salicylic acid (200 mg/dL) to urine specimens did not reproduce the negative bias, ingestion of aspirin (acetylsalicylic acid) did by -0.09 mA/min per 1 mg/dL (72.4 mumol/L) salicylate. Negative biases observed for other Emit d.a.u. assays after salicylate ingestion lead us to conclude that ingestion of therapeutic doses of aspirin may cause false-negative results for drug screens in urines by this technology. PMID: 8149618 [PubMed - indexed for MEDLINE]

08-03-2004, 01:24 AM	#6
choke-a-toke New Member Join Date: Aug 2004 Posts: 4 Grams: 1,771.15 Thanks: 0 Thanked 0 Times in 0 Posts Stock Portfolio Total Value: 0.000 Gain/Loss: 0.000%	How long did you stop I know you said this method has worked for you and others, but what I need to know is; How long did you stop smoking before you started your asprin/dilution method? I smoked sunday the 1st. and plan on not smoking again till after the test wich will be on the 10th. However, I want to smoke now. Also, what kind of smoker would you consider yourself as, Light or Heavy?

08-07-2004, 05:34 PM	#8
GanjConsultant New Member Join Date: Aug 2004 Posts: 1 Grams: 1,769.25 Thanks: 0 Thanked 0 Times in 0 Posts Stock Portfolio Total Value: 0.000 Gain/Loss: 0.000%	More recommendations I work for a large consulting firm in DC. I recently had to take a urine test in order to get security clearance for the project I'm currently working on. I wanted to thank S2 for his post on Dilution but wanted to let all of you know that there is no need to over saturate your salt levels as much as he recommends. Gatorade has more than enough salt naturally in it to replenish the salt levels in your body. In fact most trainers would recommend 1/2 water to 1/2 Gatorade as the best mixture to recover from the normal loss of salts and electrolytes caused by strenuous activities. The reason for this is that Gatorade has more salt and electrolytes in it than is needed and the excess is unnecessarily removed by your liver. Thus if you drank pure Gatorade to dilute in conjunction with the other ingredients, you wouldn't have any problem passing the urine integrity tests. No need to make people puke from such an awful tasting mixture. I would also recommend eating a meal about 3-4 hours before the test so that you are getting naturally generated vitamins, minerals, proteins, carbohydrates, and fats going through your liver on top of the other things you’re doing. Stay hydrated at normal levels up until 2 hours before the test and then follow S2's liquid intake recommendations. Finally, I would recommend taking a multivitamin about 1 hour before the test. This worked for me and I hope it can help you pass these pointless tests. Corporate pot smokers unite

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